S. Stremersch PhD, S. De Smedt , K. Braeckmans , K. Raemdonck
This report briefly discusses the use of extracellular vesicles in a pharmaceutical context. In the first part, the application of extracellular vesicles as a bio-inspired drug delivery carrier for small nucleic acid drugs was assessed. In this context, optimal purification strategies were evaluated and a new cargo loading method was developed for siRNA. In addition, the siRNA delivery performance of extracellular vesicles was bench-marked against a synthetic liposomal nanocarrier. In the second part, a new analysis platform based on Raman spectroscopy was developed to exploit extracellular vesicles as a biomarker source for liquid biopsies.